© Borgis - Postepy Fitoterapii 2, s. 66-70
*Anna Kędzia
Aktywność olejku szałwiowego wobec bakterii beztlenowych
The activity of sage essential oil (Ol. salviae) against anaerobic bacteria
Zakład Mikrobiologii Jamy Ustnej Akademii Medycznej w Gdańsku
Kierownik Zakładu: prof. dr hab. Anna Kędzia
Summary
A total of 63 strains of anaerobic bacteria isolated from samples obtained from patients with infections of oral cavity and upper respiratory tract were tested. The susceptibility (MIC) anaerobic bacteria to sage essential oil was determined by means of a plate dilution technique in Brucella Agar with 5% defibrinated sheep blood. The inoculum containing 106 cfu per spot was seeded with the Steers inoculator upon the surface of agar. Incubation was performed in anaerobic conditions at 37°C in anaerobic jars. After 48 hours the lowest concentration of sage essential oils inhibiting the growth of anaerobic bacteria was read. The results of these investigations indicated, that strains belonging to the genera of Prevotella (69%), Porphyromonas (50%), Finegoldia (100%) and Ruminococcus (100%) were the most sensitive to sage essential oil. The strains of Bacteroides and Gram-positive rods were less sensitive (29% and 33% respectively). The strains of Gram-negative anaerobic cocci belonging to genera Veillonella were the least sensitive to essential oil. The growth of these strains were inhibited in concentrations from 1 to 3 mg/ml.
Key words: anaerobes, antibacterial activity, infections, sage essential oil
Piśmiennictwo
1. Kędzia B., Segiet-Kujawa E., Hołderna E. i wsp.: Skład chemiczny oraz działanie przeciwdrobnoustrojowe olejku szałwiowego ( Ol. Salviae). Herba Pol. 1990, 4, 155. 2. Kovatcheva N., Zheljazkov V.D.: Essentials oil content and components of Salvia officinalis L. from Bulgaria. Proc. 27th Int. Symp. on Essential Oils. Sept. 8-11, Vienna 1996, 237. 3. Olszewska-Kaczyńska J., Miłkowska K: Chemical analysis of essential oil and solvents extracts of sage ( Salvia officinalis L.) plant from Poland. Proc. 27th Int. Symp. on Essential Oils. Sept. 8-11, Vienna 1996, 233. 4. Kalemba D.: Przeciwbakteryjne i przeciwgrzybowe właściwości olejków eterycznych. Post. Mikrobiol. 1998, 38, 2, 165. 5. Lutomski J., Alkiewicz J.: Leki roślinne w profilaktyce i terapii. PZWL, Warszawa 1993. 6. Kałowski M., Kędzia A.: Nieprzetrwalnikujące drobnoustroje beztlenowe. W: Diagnostyka mikrobiologiczna w medycynie (red. W. Kędzia). PZWL, Warszawa 1990. 7. Holdeman L.V., Cato E.P., Moore W.E.C.: Anaerobe Laboratory Manual. V.P.I., Blacksburg. 4th ed., Baltimore M.D., Virginia 1977. 8. Holt J.G.: Bergey´s Manual of Determinative Bacteriology. Williams and Wilkins, 9th ed. Baltimore M.D. 1993. 9. Olsen I., Shah H.N.: Review and outcome of the Meetings held Manchester U.K., June 2000, by the International Committee on the Systematic of Prokaryotes Subcommittee on the Taxonomy of Gram-negative Anaerobic Rods. Anaerobe 2001, 7, 329. 10. Ferrini A.M., Mannoni V., Hodzic S. i wsp.: Antimicrobial activity of bergamot oil in relation to chemical composition and different origin. Riv. Ital. EPPOS, 1998, 9 (Spec. Num.) 140. 11. Zani F., Massimo G., Benvenuti S. i wsp.: Studies on the genotoxic properties of essential oils with Bacillus subtilis rec-assay and Salmonella /microsome revision assay. Planta Med. 1991, 57, 237. 12. Maruzzella J.C., Sicurella N.A.: Antibacterial activity of essentials oil vapors. J. Am. Pharm. Assoc. 1960, 49, 692. 13. Hili P., Evans C.S., Veness R.G.: Antimicrobial action of essential oils: the effect of dimethylsulfoxide on the activity of cinnamoni oil. Lett. Appl. Microbiol. 1997, 24, 269. 14. Biondi D., Cianci P., Geraci C. i wsp.: Antimicrobial activity and chemical composition of essential oils from Sicilian aromatic plants. Flavour. Fragr., 1993, 8, 331. 15. Shapiro S., Meier A., Guggenheim B.: The antimicrobial activity of essential oils and essential oil components towards oral bacteria. Oral Microbiol. Immunol. 1994, 9, 202. 16. Crociani F., Biavati B., Alessandrini A. i wsp.: Growth inhibition of essentials oils and other antimicrobial agents towards Bifidobacteria from dental caries. Proc. 27th Int. Symp. on Essential Oils. Sept. 8-11, Vienna 1996, 40.
otrzymano/received: 2006-05-17
zaakceptowano/accepted: 2006-06-26
Adres/address:
*Anna Kędzia
Zakład Mikrobiologii Jamy Ustnej
Akademii Medycznej w Gdańsku
ul. Do Studzienki 38, 80-227 Gdańsk
tel. (58) 349-21-85
e-mail: zmju@amg.gda.pl
