© Borgis - Postepy Fitoterapii 2, s. 66-70
*Anna Kędzia
Ocena działania przeciwbakteryjnego olejku goździkowego ( Oleum Caryophylli)
Evaluation of antibacterial activity of clove oil (oleum caryophylli)
Katedra Mikrobiologii i Zakład Mikrobiologii Jamy Ustnej Akademii Medycznej w Gdańsku
Kierownik Katedry i Zakładu: prof. dr hab. Anna Kędzia
Summary
The sensitivity to clove oil (Avicenna-Oil) 68 strains of anaerobic bacteria isolated from patients with infections of oral cavity was determined by means of plate dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood, menadione and hemin. Inoculum containing 106 CFU per spot was seeded with Steers replicator upon the surface of agar with oil and the plate without oil (strains growth control). Incubation the plates was performed in anaerobic conditions in anaerobic jar for 48 h in 37°C. The MIC was defined as the lowest concentration of the clove oil inhibiting the growth of anaerobes. The results of investigations indicated, the most sensitive to oil from Gram-negative rods were the strains from the genera of Fusobacterium. MIC for 50% of these strains were to the concentrations within the ranges from ≤0.06 do 0.5 mg/ml. The strains from the genera of Porphyromonas were the lowest sensitive to oil. The growth of the strains were inhibited by concentrations within the ranges of 0.5-≥2.0 mg/ml. The oil was very active against the Gram-positive anaerobic bacteria. The concentrations in ranges ≤0.06-0.5 mg/ml inhibited the growth of 62% the cocci and 57% the rods.
Key words: anaerobic bacteria, infection of oral cavity, susceptibility, clove oil
Piśmiennictwo
1. Brud W.S., Konopacka I.: Pachnąca apteka. Wyd. Pagina. Warszawa 1998. 2. Chaieb K., Hajlaoni H., Zantar T. i wsp.: The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata ( Syzigium aromaticum L. Myrtaceae): A short review. Phytother. Res. 2007, 21, 501. 3. Lutomski J., Alkiewicz J.: Leki roślinne w profilaktyce i terapii. PZWL 1993. 4. Friedman M., Henika P.R., Mandrell R.E.: Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. J. Food Prot. 2002, 65, 1545. 5. Kalemba D., Kunicka A.: Antibacterial and antifungal properties of essential oils. Curr. Med. Chem. 2003, 10, 813. 6. Chami F., Chami N., Bennis S. i wsp.: Oregano and clove essential oils induce surface alternation of Saccharomyces cerevisiae. Phytother. Res. 2005, 19, 405. 7. Feres M., Figueiredo L.C., Barreto I.M. i wsp.: In vitro antimicrobial activity of plant extracts and propolis in saliva samples of healthy and periodontally involved subjects. J. Int. Acad. Periodontol. 2005, 7, 90. 8. Ogunwande I.A., Olawore N.O., Ekundayo O. i wsp.: Studies on the essential oils composition, antibacterial and cytotoxicity of Eugenia uniflora L. Int. J. Aromather. 2005, 15, 147. 9. Enzo A.P., Susan J.S.: Antibacterial activity of Australian plant extracts against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE). J. Basic Microbiol. 2002, 42, 444. 10. Boonchird C., Flegel T.W.: In vitro antifungal activity of eugenol and vanillin against Candida albicans and Cryptococcus neoformans. Can. J. Microbiol. 1982, 28, 1235. 11. Harris R.: Progress with superficial mycoses using essential oils. Int. J. Aromather. 2002, 12, 83. 12. Velluti C., Sanchis V., Ramos A.J. i wsp.: Impact of essential oil on growth rate, zearalenone and oleoxynivalenol production by Fusarium graminearum under different temperature and water activity condition in maize grain. J. Appl. Microbiol. 2004, 96, 716. 13. Pawar V.C., Thaker V.S.: In vitro efficacy of 75 essential oils against Aspergillus niger. Mycoses 2006, 49, 316. 14. Inouye S.: Laboratory evaluation of gaseous essential oils (part 1). Int. J. Aromather. 2003, 13, 2/3, 95. 15. Hussein G., Miyashiro H., Nakamura N. i wsp.: Inhibitory effects of Sudanese medical plant extracts on hepatitis C virus (HCV) protease. Phytother. Res. 2000, 14, 510. 16. Burt S.A., Reinders R.D.: Antibacterial activity of selected plant essential oils against Escherichia coli 0157:H7. Lett. Appl. Microbiol. 2003, 36, 162. 17. Kurokawa M., Hozumi T., Basnet P. i wsp.: Purification and characterization of eugeniin as an anti-herpesvirus compound from Geum japonicum and Syzygium aramaticum. J. Pharmacol. Exp. Ther. 1998, 284, 728. 18. Larhsini M., Oumoulid L., Lazarek H.B. i wsp.: Antibacterial activity of some Maroccan medicinal plants. Phytother. Res. 2001, 15, 250. 19. Perez C., Agnese A.M., Cabrera J.L.: The essential oil of Senecio graveolens ( Compositae): chemical composition and antimicrobial activity tests. J. Ethnopharmacol. 1999, 66, 91. 20. Bagamboula C.F., Uyttendaele M., Debevere J.: Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiol. 2004, 21, 33. 21. Zaika L.L.: Species and herbs: their antimicrobial activity and its determination. J. Food Nutr. 1988, 9, 97. 22. Smith-Palmer A., Steward J., Fyte L.: Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Lett. Appl. Microbiol. 1998, 26, 118. 23. Cox S.D., Mann C.M., Markham J.L.: Interactions between compounds of the essential oil of Melaleuca alternifolia.J. Appl. Microbiol. 2001, 91, 492. 24. Ogata M., Hoshi M., Urano S., Endo T.: Antioxidant activity of eugenol and related monomeric and dimeric compounds. Chem. Pharm. Bull. 2000, 48, 1467. 25. Gulcin I., Gungor I., Beydemir S.S., i wsp.: Comparison of antioxidant activity of clove ( Eugenia caryophyllata Thumb.) buds and lavender ( Lavandula stoechas L.). Food Chem. 2004, 87, 393. 26. Zheng G.Q., Kenney P.M., Lam L.K.T.: Sesquiterpenes from clove ( Eugenia caryophyllata). J. Nat. Prid. 1992, 55, 999. 27. Yoo C.B., Han K.T., Cho K.S. i wsp.: Eugenol isolated from the essential oil of Eugenia caryophyllata induces a reactive oxygen species-mediated apoptosis in HL-60 human promyelocytic leukemia cells. Cancer Lett. 2005, 225, 41. 28. Yang Y.C., Lee S.H., Lee W.I. i wsp.: Ovicidal and adulticidal effects of Eugenia caryophyllata bud and leaf oil compounds on Pediculus capitis. J. Agricult. Food Chem. 2003, 51, 4884. 29. Trongtohit Y., Rongsiyam Y., Komalamisra N., Apiwathnasorn C.: Comparative repellency of 38 essential oils against mosquito bites. Phytother. Res. 2005, 19, 303. 30. Holdeman L.V., Cato E.P., Moore W.E.C.: Anaerobe Laboratory Manual. V.P.I. Blacksburg 4th ed. Baltimore M.D., Virginia 1977. 31. Kałowski M., Kędzia A.: Nieprzetrwalnikujące drobnoustroje beztlenowe. W: Diagnostyka mikrobiologiczna w medycynie. (red. W. Kędzia). PZWL, Warszawa 1990. 32. Holt J.G.: Bergeys Manual of Determinative Bacteriology. Williams and Wilkins ed. 9th ed. Baltimore M.D. 1993. 33. Forbes B.A., Sahm D.F., Weissfeld A.S.: Bailey and Scott´s Diagnostic Microbiology. 12th ed. Mosby Elsevier. St. Louis 2007. 34. Murdoch D.A., Shah H.N.: Reclassification of Peptostreptococcus magnus (Prevot 1933), Holdeman and Moore 1972 as Finegoldia magna comb. nov. and Peptococcus micros (Prevot 1933) Smith 1957 as Micromonas micros comb. nov. Anaerobe 1999, 5, 555. 35. Olsen I., Shah H.N.: Review and outcome of the Meetings held Manchester U.K., June 2000, by the Intarnational Committee on the Systematic of Prokaryotes Subcommittee on the Taxonomy of Gram-negative Anaerobe Rods. Anaerobe 2001, 7, 329. 36. Saeki Y., Ito Y., Shibata M. i wsp.: Antimicrobial action of natural substances on oral bacteria. Bull Tokyo Dent. Coll. 1989, 30, 129. 37. Grociani F., Biavati B., Alessandrini A., Zani G.: Growth inhibition of essential oils and other antimicrobial agents towards Bifidobacterium from dental caries. 27th Int. Symp. on Essential Oils. 1996, Vienna, 40. 38. Ismail A.A., Pierson M.D.: Effect of sodium nitrite and origanum oil on growth and toxin production Clostridium botulinum in TYG broth and ground pork. J. Food Prot. 1990, 53, 958.
otrzymano/received: 0000-00-00
zaakceptowano/accepted: 2007-06-08
Adres/address:
*Anna Kędzia
Zakład Mikrobiologii Jamy Ustnej Akademii Medycznej w Gdańsku
ul. Do Studzienki 38, 80-227 Gdańsk
tel. (0-58) 349-21-85
e-mail: zmju@amg.gda.pl
