© Borgis - Postepy Fitoterapii 3, s. 147-151
*Anna Kędzia1, Andvzej W. Kędzia2
Działanie oleju kamforowego na bakterie beztlenowe
ACTIVITY OF CAMPHOR OIL TO ANAEROBIC BACTERIA
1Zakład Mikrobiologii Jamy Ustngj, Katedra Mikrobiologii Gdańskiego Uniwersytetu Medycznego
Kierownik Zakładu i Katedry: dr hab. Anna Kędzia, prof. ndzw.
2II Katedra Pediatrii, Klinika Endokrynologii i Diabetologii Wieku Rozwojowego Uniwersytetu Medycznego"w Poznaniu
Kierownik Katedry: prof. UM dr hab. n.med. Marek Niedziela
Summary
The sensitivity to camphor oil of 67 strains of anaerobic bacteria isolated from infections of oral cavity and respiratory tract were tested. The components of camphor oil was: camphora (10 parts) and rape oil (90 parts). The susceptibility of anaerobes was performed by means of plate dilution technique in Brucella agar with added 5% defibrynated sheep blood, menadione and hemin. The inoculum containing 105 CFU per spot was seeded Steers replicator upon the surface of the agar with and without the oil (strains growth control). Incubation was performed at 37°C for 48 hrs in anaerobic jars under anaerobic conditions. The MIC was defined as the lowest oil concentrations that completely inhibited the growth of anaerobic bacteria. The results indicated that the most susceptible to camphor oil, from Gram-negative bacteria, were the strains from genera Prevotella and Porphyromonas. MIC´s for 42% and 40% of these strains were in the ranges Ł 0,12-0,5 mg/ml. The strains from the genera of Bacteroides and Veillonella were the lowest sensitive to tested oil (MIC in range of 1,0 – ł 4,0 mg/ml). The Gram-positive anaerobes were the most sensitive to camphor oil. The growth of 85% of these strains were inhibited by low concentrations within the range of Ł 0,12-0,5 mg/ml. The Gram-positive cocci were the more susceptible (90%) than Gram-positive rods (80%). The camphor oil was most effective against Gram-positive than Gram-negative anaerobes.
Key words: anaerobic bacteria, camphor oil, infection oral cavity, respiratory tract, sensitivity
Piśmiennictwo
1. Brud WS, Konopacka J. Pachnąca apteka. Wyd. Pagina, Warszawa 1998. 2. Chelliah DA. Biological activity prediction of an ethno medicinal plant Cinnamomum camphora through bio-informatics. Ethnobotanical Leaflets 1998; 12:181-90. 3. Wan-yang S, Wei H, Guang-yu W i wsp. Study on chemical constituents of the essential oil and classification of types from Cinnamomum camphora. Acta Botanica Sinica 1989; 31:209-14. 4. Kumar M, Ando Y. Carbon nanotubes from camphor: An environment- friendly nanotechnology. International Conference on Nanoscience and Technology (INC and T 2006). J Physics: Conference Series 2007; 61:643-6. 5. Inouye S, Uchida K, Maruyama N i wsp. A novel metod to estimate the contribution of the vapor activity of essential oils in agar diffusion assay. Nippon Ishinhin Gakkai Zasshi 2006; 47:91-8. 6. Lis-Balchin M, Deans SG, Eaglesham E. Relationship between bioactivity and chemical composition of commercial essential oils. Flavour Frag J 1998; 13(20):98-104. 7. Takaoka D, Takaoka K, Ohshita T, Hiroi M. Sesquiterpene alcohols in camphor oil. Phytochem 1976; 15:425-6. 8. Sattar A, Gilani AM, Sead MA. Gas chromatographic examination of the essential oil of Cinnamomum camphora. Pak J Sci Ind Res 1991; 34:135-6. 9. Mishra K, Dwivedi SK, Kishoe N i wsp. Fungistatic properties of essential oil Cinnamomum camphora. Int J Pharmacog 1991; 29:259-62. 10. Lis-Balchin M. Int J Aromather 1996; 29(3):43. 11. Mastura M, Azar M, Khozirah S i wsp. Anticandidal and antidermatophytic activity of Cinnamomum species essential oils. Cynobios 1999; 98:17-23. 12. Srivastava B, Singh P, Shukla R i wsp. A novel combination of the essential oils of Cinnamomum camphora and Alpinia galanga in checking aflatoxin B1 production by a toxigenic strain of Aspergillus flavus. World J Microbiol Biotechnol 2008; 24:693-7. 13. Lin CH, Mishra AK, He B i wsp. Composition and antifungal activity of essential oils from Artemisia princeps and Cinnamomum camphora. Intern Pest Control 2001; 43(2):72-4. 14. De Medici D, Pieretti S, Salvatore G i wsp. Chemical analysis of essential oil of Malgasy medicinal plants by gas chromatography and NMR spectrometry. Flav Frag J 1992; 7:275-81. 15. Friedman M, Henika PR, Mandrell RE. Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes and Salmonella enterica. J Food Protect 2002; 65:1545-60. 16. Morris JA, Khettry A, Seitz EW. Antimicrobial activity of aroma chemicals and essential oils. J Am Oil Chem Soc 1979; 56:595-603. 17. Vidya TJ, Vidya P. Antimicrobial activity of Scavon Vet Cream. Veterinarian 2000; 24:16-22. 18. Holdeman LV, Cato EP, Moore WEC. Anaerobe Laboratory Manual V.P.I. Blacksburg 4th ed. Baltimore M.D., Virginia 1977. 19. Kałowski M, Kędzia A. Nieprzetrwalnikujące drobnoustroje beztlenowe. W: Diagnostyka mikrobiologiczna w medycynie. (red. W. Kędzia) PZWL, Warszawa 1990. 20. Holt JG. Bergeys´ Manual of Determinative Bacteriology. Williams and Wilkins ed. 9th ed. Baltimore 1993. 21. Forbes BA, Sahn DF, Weissfeld AS. Bailey and Scott´s Diagnostic Microbiology. 12th ed. Mosby Elsevier. St,Louis 2007. 22. Cardullo MA, Gilroy JJ. Growth inhibition of Escherichia coli strain 82/r by d-camphor. Cand J Microbiol 1973; 19:1015-19. 23. Cardullo MA, Gilroy JJ. Inhibition of oxidative metabolism Escherichia coli by d-camphor and restoration of oxidase activity by quinines. Cand J Microbiol 1975; 21:1357-61. 24. Adhikari PC. Growth inhibition of Vibrio cholerae by d-camphor. J General Microbiol 1975; 91:414-6.
otrzymano/received: 2009-09-30
zaakceptowano/accepted: 2009-10-15
Adres/address:
*Anna Kędzia
Zakład Mikrobiologii Jamy Ustnej
Katedra Mikrobiologii GUMed
ul. Do Studzienki 38, 80-227 Gdańsk
tel.: (058) 349-21-85
e-mail: zmju@amg.gda.pl
