© Borgis - Postepy Fitoterapii 1, s. 19-23
*Anna Kędzia1, Andrzej W. Kędzia2
Działanie in vitro olejku sosnowego wobec bakterii beztlenowych wyizolowanych z jamy ustnej i dróg oddechowych
ACTIVITY IN VITRO OF PINE OIL (OLEUM PINI SILVESTRIS) AGAINST ANAEROBIC BACTERIA ISOLATED FROM ORAL CAVITY AND UPPER RESPIRATORY TRACT
1Zakład Mikrobiologii Jamy Ustnej, Katedra Mikrobiologii Akademii Medycznej w Gdańsku
Kierownik Zakładu i Katedry: dr hab. Anna Kędzia, prof. ndzw.
2II Katedra Pediatrii, Klinika Endokrynologii i Diabetologii Wieku Rozwojowego Uniwersytetu Medycznego w Poznaniu
Kierownik Katedry: dr hab. n. med. Marek Niedziela, prof. UM
Summary
In the present study, pine oil (Oleum pini silvestris) were investigated for activity against 98 anaerobic bacteria using plate dilution technique method in Brucella agar. The inoculum contained 106 CFU per spot. The agar plate were inoculated using a Steers replicator. Incubation the plater was performer in anaerobic jars in 37°C for 48 hrs. The MIC was interpreted as the lowest concentrations of pine oil completely inhibiting the growth of anaerobes. The results indicated that the most susceptible to tested aetheric oil from Gram-negative rods were the strains from the genera of Prevotella and Porphyromonas. MIC´s for 55% and 47% of these strains respectively, were to the concentrations within the range from ≤ 0.03 to 0.5 mg/ml. The strains from the genera of Bacteroides, particularly from species of B. fragilis were the lowest sensitive (MIC in ranges 0.25 ≥ 2.0 mg/ml). The strains of Gram-negative cocci from the genera of Veillonella were the most resistance (MIC 1.0-≥ 2.0 mg/ml). The Gram-positive rods and cocci showed similar susceptibility to pine oil. Lower concentrations in ranges ≤ 0.03-0.5 mg/ml inhibited the growth of 44 and 42% these bacteria respectively.
Key words: pine oil, anaerobes, susceptibility, oral cavity, respiratory tract
Piśmiennictwo
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otrzymano/received: 2009-03-10
zaakceptowano/accepted: 2009-03-26
Adres/address:
*Anna Kędzia
Zakład Mikrobiologii Jamy Ustnej
Katedra Mikrobiologii AM w Gdańsku
ul. Do Studzienki 38, 80-227 Gdańsk
tel. (0-58) 349-21-85
e-mail: zmju@amg.gda.pl
