© Borgis - Postepy Fitoterapii 3, s. 134-138
*Anna Kędzia1, Andrzej W. Kędzia2
Działanie preparatu Amol na grzyby drożdżopodobne wyizolowane z jamy ustnej i dróg oddechowych
Activity of amol against yeast-like fungi isolated from oral cavity and respiratory tract
1Zakład Mikrobiologii Jamy Ustnej, Katedra Mikrobiologii Akademii Medycznej w Gdańsku
Kierownik Zakładu i Katedry: prof. ndzw. dr hab. Anna Kędzia
2II Katedra Pediatrii, Klinika Endokrynologii i Diabetologii Wieku Rozwojowego Uniwersytetu Medycznego w Poznaniu
Kierownik Katedry: prof. UM dr hab. n. med. Marek Niedziela
Summary
A total of 106 strains of yeast-like fungi isolated from oral cavity or respiratory tract and 2 standards strains were tested. The susceptibility (MIC) yeast-like fungi to Amol was determined by means of plate dilution technique in Sabouraud agar. The inoculum containing 106 CFU per spot was seeded with Steers inoculator upon the surface of agar containing various drug concentrations. Incubation was performed at 37°C for 24 or 48 h (for slow growth strains) in aerobic conditions. The MIC was interpreted as the minimal Amol concentration that completely inhibited growth of yeast. The results of these investigations indicated that the most susceptible to Amol were the strains belonging to the genera of Candida, from genus of Candida mesenterica, from genus Rhodotorula rubra and Saccharomyces cerevisiae (MICs for 100% of strains were ≤ 2,5 to 5,0 mg/ml). The strains of Candida albicans were less sensitive. The growth of 40% of these strains were inhibited by Amol within the range from ≤ 2,5 to 15,0 mg/ml. But for 27% strains MIC was> 20,0 mg/ml. The strains of Candida dubliniensis were the lowest sensitive (MIC ≥ 20,0 mg/ml).
Key words: yest-like fungi, amol, antifungal activity, infections, oral cavity, respiratory tract
Piśmiennictwo
1. Kleinegger CL, Lockhart SR, Vargas K, Soll DR. Frequency, intensity, species and strains of oral Candida vary as a function of host age. J Clin Microbiol 1996; 34: 2246. 2. Hossain H, Ansari F, Schulz-Weidner N i wsp. Clonal identity of Candida albicans in the oral cavity and the gastrointestinal tract of pre-school children. Oral Microbiol Immunol 2003; 18: 301. 3. Epstein JB, Truelove EL, Izutzu KT. Oral candidiasis: pathogenesis and host defence. Rev Infect Dis 1984; 1: 96. 4. Batura-Gabryel H.: Poszukiwanie grup ryzyka zakażenia Candida i kandydozy jamy ustnej wśród chorych na przewlekłe choroby układu oddechowego. Mik lek 2000; 7: 133. 5. Belazi M, Velegrain A, Fleva A i wsp. Candidal overgrowth in diabetic patients: potential predisposing factors. Mycoses 2005; 48: 192. 6. Mc Mullan-Vogel CG, Jude HD, Ollert MW i wsp. Serotype distribution and secretory acid proteinase activity of Candida albicans isolated from the oral mucosa of patients with denture stomatitis. Oral Microbiol Immunol 1999; 14: 183. 7. Hannula J, Saarela M, Jousimies-Sommer H i wsp. Age-related acquisition of oral and nosopharyngeal yeast species and stability of colonization in young children. Oral Microbiol Immunol 1999; 14: 176. 8. Kunar BV, Padshetty NS, Bai KY i wsp. Prevalence of Candida in the oral cavity of diabetes subjects. J A P J 2005; 53: 599. 9. Radford DR, Challacombe SJ, Walter JD. Denture plaque and adherence of Candida albicans in denture- base materials in vivo and in vitro. Crit Rev Oral Biol Med 1999; 10: 99. 10. Webb BC, Thomas CJ, Willcox MD i wsp. Candida - associated denture stomatitis. Aetiology and management a review. Part 1. Factors influencing distribution of Candida species in the oral cavity. Aust Dent J 1998; 43: 45. 11. Dorko E, Jenca A, Pilipcinec E i wsp. Candida-associated denture stomatitis. Folia Microbiol (Praha) 2001; 46: 443. 12. Sugar AM, Lymann CA. Candida.W: A practical guide to medically important fungi and diseases they cause. Lippincott-Raven, Philadelphia. 1977; 34. 13. Richartson MD, Warnock DW. Fungal infection, diagnosis and management. Blackwell Scientific Publ Oxford 1993. 14. Cannon RD, Holmes AR, Mason AB i wsp. Oral Candida: clearance, colonization or candidiasis? J Dent Res 1995; 74: 1152. 15. Nawrot U, Grzybek-Hryncewicz K, Karpiewska A. Podatność grzybów z rodzaju Candida na działanie antymikotyków oznaczona metodą rozcieńczeniową. Mik Lek 2000; 7: 19. 16. Kuriyama T, Williams DW, Bagg J i wsp. In vitro susceptibility of oral Candida to seven antifungal agents. Oral Microbiol Immunol 2005; 20: 349. 17. Martin-Mazuelos E, Aller AJ, Romero MJ i wsp. Response to fluconazole and itraconazole of Candida spp. in denture stomatitis. Mycoses 1997; 40, 7-8: 283. 18. Hitchcock CA, Pue GW, Troke PF i wsp. Fluconazole resistance in Candida glabrata. Antimicrob Agents Chemother 1993; 37: 1962. 19. Rex JH, Rinaldi MG, Pfaller MA. Resistance of Candida species to Fluconazole. Antimicrob Agents Chemother 1995; 39: 1. 20. Kalemba D. Przeciwbakteryjne i przeciwgrzybowe właściwości olejków eterycznych. Post Mikrobiol 1998; 38: 185. 21. Morris JA, Khettry A, Seitz EW. Antimicrobial activity of aroma chemicals and essential oils. J Am Oil Chem Soc 1979: 56: 995. 22. Cowan MW. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12: 564. 23. Kalemba D, Kunicka A. Antibacterial and antifungal properties of essential oils. Curr Med Chem 2003; 10: 813. 24. Pauli A. Antimicrobial properties of essential oil constituents. Int J Aromather 2001; 11: 126. 25. Yousef RT, Tawil GG. Antimicrobial activity of volatile oils. Pharmazie 1980; 35: 698. 26. Saeki Y, Ito Y, Shibata M i wsp. Antimicrobial action of natural substances on oral cavity bacteria. Bull Tokyo Dent Coll 1989; 30: 129. 27. Inouye S, Takizawa T, Yamaguchi H. Antibacterial activity and their major constituents against respiratory tract pathogens by gaseous contact. J Antimicrobial Chemother 2001; 47: 565. 28. Chaieb K, Hajiaoni H, Zmantar T i wsp. The chemical composition and biological activity of clove essential oil, Eugenia caryophylata ( Syzygium aromaticum L., Myrtaceae): A short review. Phytother Res 2007; 21: 501. 29. Inouye S. Laboratory evaluation of gaseous essential oils. (part 1) Int J Aromather 2003; 13: 95. 30. Megalla SE, El-Keltawi NEM, Ross SA. A study of antimicrobial action of some essential oil constituents. Herba Pol 1980; 3:181. 31. Griffin SG, Wyllie SG, Markhan JL i wsp. The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flav Fragr J 1999; 14: 322. 32. Chami N, Bennis S, Chami F i wsp. Study of anticandidal activity of carvacrol and eugenol in vitro and in vivo. Oral Microbiol Immunol 2005; 20: 106. 33. Harris R. Progress with superficial mycoses using essential oils. Int J Artomather 2002; 12: 83. 34. Singh HB, Srivastova M, Singh AB i wsp. Cinnamon bark oil a potent fungitoxicant against fungi causing respiratory tract mycoses. Allergy 1995; 50: 995. 35. D´Auria FD, Tecca M, Strippoli V i wsp. Antifungal activity of Lavandula angustifolia essential oil against Candida albicans yeast and mycelia form. Med Mycol 2005; 43: 391. 36. Maruzzella JC, Liguori L. The in vitro antifungal activity of essential oils. J Am Pharm Assoc 1956; 47: 250. 37. Kędzia B, Hołderna-Kędzia E. Badania wpływu olejków eterycznych na bakterie, grzyby i dermatofity chorobotwórcze dla człowieka. Post Fitoter 2007; 2: 71. 38. Kędzia B i wsp. Skład i działanie przeciwdrobnoustrojowe olejku melisowego i jego składników. Herba Pol 1994; 40: 5. 39. Sorensen JM. Mellissa officinalis. Int J Aromather 2000; 10: 7. 40. Pattnaik S, SubramanyanVR, Bapaji M i wsp. Antibacterial and antifungal activity of aromatic constituents of essential oils. Microbios 1997; 89: 39.
otrzymano/received: 2008-09-12
zaakceptowano/accepted: 2008-09-27
Adres/address:
*Anna Kędzia
Zakład Mikrobiologii Jamy Ustnej
Katedra Mikrobiologii AM w Gdańsku
ul. Do Studzienki 38, 80-227 Gdańsk
tel. (058) 349-21-85
e-mail: zmju@amg.gda.pl
