© Borgis - Medycyna Rodzinna 3, p. 66-68
1Joanna Wróblewska*, 1Eugenia Gospodarek, 1Małgorzata Bredow, 2Alina Grzanka, 2Alicja Niewińska
L-form Staphylococcus hominis wywołane pod wpływem ampicyliny i cefazoliny
L-form of Staphylococcus hominis induced by ampicilin and cefazolin
1Katedra i Zakład Mikrobiologii Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy
Uniwersytetu Mikołaja Kopernika w Toruniu
Kierownik Katedry i Zakładu: prof. UMK dr hab. n. med. Eugenia Gospodarek
2Katedra i Zakład Histologii i Embriologii Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy
Uniwersytetu Mikołaja Kopernika w Toruniu
Kierownik Katedy i Zakładu: prof. UMK dr hab. Alina Grzanka
Summary
S. hominis L-forms have not been studied carefully yet. They do not grow on routinely used microbiological media but they can be cultured on special hypertonic medium. No information on in vitro induction and evaluation of S. hominis L-forms on solid media can be found in literature.
Material and methods. 36 S. hominis strains isolated from patients of dr. A. Jurasz University Hospital in Bydgoszcz were analysed. These strains were identified with the API Staph (bioMérieux) and tested for L-forms induction with method described by Owens (1988).
Results. The studies indicated that 13.9% and 13.9% strains of S. hominis respectively changed into revert L-forms by ampicilin and cefazolin. It was observed that after 10 minutes and 24 hours incubation in BHI containing ampicilin 11.1% and 13.9% strains of S. hominis changed into revert L-forms respectively. Whereas the cefazolin was used 13.9%, 8.3% of strains of S. hominis changed into revert L-forms during 10 minutes and 24 hours of incubation in BHI respectively.
Conclusion. Ampicilin and cefazolin are effective antibiotics which induce L-forms of S. hominis strains. This study suggest that the time of S. hominis strains incubation in BHI with antibiotics did not play a significant role in changing these bacteria into L-forms.
Key words: ampicilin, cefazolin, L-forms, S. hominis
Pi¶miennictwo
1. Crossley KB, Archer GL: The staphylococci in human disease. Churchill Livingstone Inc. New York: 1997. p. 119-22. 2. Huebner J, Goldmann DA: Coagulase-negative staphylococci: role as pathogen. Annu Rev Med 1999; 50: 223-36. 3. Etienne J, Eykyn SJ: Increase in native valve endocarditis caused by coagulase-negative staphylococci: an anglo-french clinical and microbiological study. Br Heart J 1990; 64: 381-4. 4. Sunbul M, Demirag MK, Yilmaz O, Yilmaz H, Ozturk R, Leblebicioglu H: Pacemaker lead endocarditis caused by Staphylococcus hominis.PACE 2006; 29: 543-5. 5. von Eiff C, Proctor RA, Peteres G: Coagulase-negative staphylococci. Postgrad Med 2001; 110: 63-6. 6. Calnen G, Campognone P, Peter G: Coagulase-negative staphylococcal bacteremia in new-borns. Clin Pediatr 1984; 23: 542-4. 7. Khadilkar V, Tudenhop D, Fraser S: A prospective study of nosocomial infections in a neonatal intensive care unit. J Pediatr Child Health 1995; 31: 387-91. 8. Giurguitzowa B, Chankova D, Zozikov B: The Staphylococci as uropathogens, frequency of isolation in hospitalized patients and susceptibility to antimicrobial agents. Ann Urol 2002; 36: 341-7. 9. Bannerman TL, RhodenDL, McAllister SK, Miller JM, Wilson LA: The source of coagulase-negative staphylococci in the endophthalmitis vitrectomy study. Arch Ophthalmol 1997; 115: 357-61. 10. Iyer MN, Wirostko WJ, Kim SH, Simons KB: Staphylococcus hominis endophthalmitis associated with capsular hypopyon. Am J Ophthalmol 2005; 139: 930-2. 11. Manikandan P, Bhaskar M, Revathy R, John RK, Narendran K, Narendran V: Speciation of coagulase negative staphylococcus causing bacterial keratitis. Indian J Ophthalmol 2005; 53: 59-60. 12. Cantwell AR. Cell-wall-deficient bacteria as a possible cause of basal cell carcinoma. JOIMR 2003; 1: 1. 13. Onwuamaegbu ME, Belcher RA, Soare C: Cell wall-deficient bacteria as a causa of infections: a review of the clinical significance. J Int Med Rse 2005; 33: 1-20. 14. Jakubczak A, Sachanowicz J, Kleczkowski M, Bukowski K: Wpływ wybranych antybiotyków na wytwarzanie from L szczepów Staphylococcus aureus. Medycyna Wet 2002; 58: 807-9. 15. Owens WE. Evaluation of various antibiotics for induction of L-form Staphylococcus aureus strains isolated from bovine mastitis. J Clin Microbiol 1988; 26: 2187-90. 16. Sachs L. Applied Statistics. Springer-Verlg. Berlin-Heidelberg. 1984. 17. Dominugue GJ, Thomas R, Walters F, Serrano A, Heidgerd PM: Cell wall deficient bacteria as a cause of idiopathic hematuria. J Urol 1993; 150: 483-5. 18. Dominigue GJ, Hellstrom WJG: Prostatis. Clin Microbiol Rev 1998; 11: 604-13. 19. Göksu N, Ataođlu H, Kemalođlu YK, Ataođlu O, Ozsökmen D, Akyildiz N: Experimental otitis media induced by coagulase negative staphylococcus and its L-forms. Int J Pediatr Otorhinolaryngol 1996; 37: 201-16. 20. Dominigue GJ, Woody HB: Bacterial persistence and expression of disease. Clin Microbiol Rev 1997; 10: 320-44. 21. Vuurde JWL, van Elvira-Recuenco M: Efficiency of procedures for induction and cultivation of Pseudomonas syringae pv. pisi L-form. Microbiol Res 2003; 158: 271-9.
otrzymano/received: 2008-07-16
zaakceptowano/accepted: 2008-08-21
Adres/address:
*Joanna Wróblewska
Uniwersytet Mikołaja Kopernika w Toruniu
Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy
Katedra i Zakład Mikrobiologii
ul. M. Skłodowskiej-Curie 9
85-094 Bydgoszcz
tel.: (0-52) 585 44 80
e-mail: joannawroblewska@wp.pl
